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CBSE Class 9 Answered

What is a black reaction?

How was Camilio Golgi able to identify it

Asked by mukunda | 02 Jan, 2020, 09:40: PM
Expert Answer
  • Black reaction is a silver staining technique which was first done by Camillo Golgi. It helped to study about the nerve cells. In this technique, cells are stained using potassium dichromate and silver nitrate.
  • In 1873, Camillo Golgi used the chemical potassium dichromate to harden the tissue.
  • However, after hardening the tissue, Golgi used a different chemical to dye the neurons. Instead of hematoxylin or carmine, Golgi submerged the tissue in a silver nitrate solution, soaking it for one to two days.
  • The silver nitrate solution reacted with the potassium dichromate and formed fragments of silver chromate on the cell membrane. Those fragments were black, hence the name black reaction.
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