CBSE Class 12-science Answered

For this purpose, Enzyme has a region where substrate molecules bind and undergo a chemical reaction.

Mechanism of Enzyme Action

• Substrate binds to the active site and fits itself perfectly into the active site.
• The binding induces little alternation in the shape of the enzyme so that the substrate can fit more tightly.
• The active site once in close proximity of the substrate breaks the chemical bonds of the substrate, and a new enzyme-product complex is formed.
• Once the new bonds are formed, the enzyme releases the product molecule.
• The free enzyme is again ready to bind to another molecule of the substrate.
• The active site is always specific with the reactive site present on the substrate.