What is genetic engineering? List the steps involved in rDNA technology.
Asked by Topperlearning User | 12th Jun, 2014, 09:24: AM
Genetic engineering involves techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus, change the phenotype of the host organism.
Steps in rDNA technology:
(i) Isolation of DNA
(ii) Fragmentation of DNA by restriction endonucleases
(iii) Isolation of the desired DNA fragments
(iv) Amplification of gene of interest
(v) Ligation of the DNA fragment into a vector using DNA ligase
(vi) Transfer of recombinant DNA into the host organism
(vii) Culturing the host cell on a suitable medium on a large scale
(viii) Extraction of the desired product
(ix) Downstream processing of the products as finished products are ready for marketing
Answered by | 12th Jun, 2014, 11:24: AM
- recombinant DNA tecnology
- please answer
- What is ori? state its importance during cloning of a vector?
- What is the host that produces a foreign gene product called? What is this product called?
- State the uses of cloning vector in biotechnology.
- Who developed the technique of electrophoresis? State its principle.
- Explain any two methods of vectorless gene transfer.
- How can DNA segments be separated by gel electrophoresis, visualised and isolated?
- Study the linking of DNA fragments shown below: (i) Name 'a' DNA and 'b' DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments.
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