Explain any two methods of vectorless gene transfer.
Asked by Topperlearning User | 12th Jun, 2014, 09:24: AM
The two methods of vectorless gene transfer are as shown below:
(i) Microinjection: The technique of introducing foreign DNA into a target cell by injecting the DNA directly into the nucleus with the help of a micro-needle is called micro-injection.
(ii) Electroporation: The process in which transient holes are produced in the plasma membrane of the target cell to incorporate foreign DNA is called electroporation.
Answered by | 12th Jun, 2014, 11:24: AM
- recombinant DNA tecnology
- please answer
- What is ori? state its importance during cloning of a vector?
- What is the host that produces a foreign gene product called? What is this product called?
- State the uses of cloning vector in biotechnology.
- Who developed the technique of electrophoresis? State its principle.
- What is genetic engineering? List the steps involved in rDNA technology.
- How can DNA segments be separated by gel electrophoresis, visualised and isolated?
- Study the linking of DNA fragments shown below: (i) Name 'a' DNA and 'b' DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments.
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