NEET Class neet Answered
when the transformant having recombinant gene will be grown in tetracycline medium then it will die. in this way can distinguish transformant from non-transformant. but transformant have already died then how they will be further used in the production of products?
Asked by rohitraman1115 | 24 Jan, 2020, 03:34: PM
Expert Answer
- Some genes called selectable markers help in selecting those host cells which contain the transformed vectors and eliminate the non-transformants.
- Transformation is a process through which a piece of DNA is introduced in a host bacterium.
- Generally, the genes encoding resistance to antibiotics such as tetracycline, ampicillin and kanamycin are useful selectable markers for E. coli.
- Plasmid pBR322 has two resistance genes—ampicillin resistance (ampR) and tetracycline resistance (tetR)—which are considered useful for selectable markers.
- Insertion of the DNA fragment into the plasmid using enzyme PstI or PvuI places the DNA insert within the gene ampR, which makes ampR non-functional.
- Bacterial cells containing such a recombinant pBR322 will be unable to grow in the presence of ampicillin but will grow on tetracycline.
- These recombinants can then be used for the production of useful products.
Answered by Sheetal Kolte | 29 Jan, 2020, 11:08: AM
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Asked by rohitraman1115 | 24 Jan, 2020, 03:34: PM
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