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Tools Of Recombinant Dna Technology Free Doubts and Solutions

CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Why is it essential to have a selectable marker in a cloning vector?

Asked by pksunilnair 12th December 2017, 8:50 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

What are recombinant DNA and transformable &non transformant DNA?

Asked by bijuaylara111 21st October 2017, 9:20 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

question test of biotech.

Asked by akshay.beloshe 12th October 2017, 7:32 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

WHAT TYPE OF QUESTIONS ARE EXPECTED IN KVPY EXAM? WHAT SHOULD I PREPARE? WILL IT BE EASY? I DON'T HAVE BIOLOGY... SO NO IDEA...PLEASE HELP...

Asked by Kalita Padmanath 19th August 2017, 2:27 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

sir what is insertional  inactivation

Asked by anu Mishra 18th March 2016, 1:36 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Explain ecological pyramids

Asked by raginigupta445 20th January 2016, 5:01 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

based on the interaction of EcoR1 with DNA, do you expect it to be a homodimer or heterodimer?

Asked by haider.samina 30th January 2015, 1:26 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Why restriction enzymes cut DNA a little away from the centre of pallindromic site

Asked by Parinita Dolly 9th January 2015, 7:05 PM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

(a) Identify A and B illustrations in the following:       (b) Write the term given to A and C, and why? (c) Expand PCR. Mention its importance in biotechnology.

Asked by Topperlearning User 12th June 2014, 9:40 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

(i) Describe the characteristics that a cloning vector must possess. (ii) Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made 'competent' to take up recombinant DNA from the medium?

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

What is a cloning vector? Explain the technique of using such a vector in E.coli.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Explain the importance of (a) ori, (b) ampR and (c) rop in the E. coli vector shown below:                            

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Study the diagram given below and answer the questions that follow:                 (a) Why have DNA fragments in band 'D' moved farther away in comparison to those in band 'C'? (b) Identify the anode end in the diagram. (c) How are these DNA fragments visualised?  

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

What is Ti plasmid? Name the organism where it is found. How does it help in genetic engineering?

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

(a) What are molecular scissors? Give one example. (b) Explain their role in recombinant DNA technology.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Study the linking of DNA fragments shown below:       (i) Name 'a' DNA and 'b' DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

How can DNA segments be separated by gel electrophoresis, visualised and isolated?

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

What is genetic engineering? List the steps involved in rDNA technology.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Explain any two methods of vectorless gene transfer.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

Who developed the technique of electrophoresis? State its principle.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

State the uses of cloning vector in biotechnology.

Asked by Topperlearning User 12th June 2014, 9:24 AM
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CBSE - XII Science - Biology - Biotechnology : Principles and Processes

What is the host that produces a foreign gene product called? What is this product called?

Asked by Topperlearning User 12th June 2014, 9:24 AM
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