reproduction in yeast,amoeba

Asked by harsha kosta | 12th Oct, 2010, 06:14: PM

Expert Answer:

Dear Student,

Yeasts have asexual and sexual reproductive cycles. The most common mode of vegetative growth in yeast is asexual reproduction by budding. Here a small bud, or daughter cell, is formed on the parent cell. The nucleus of the parent cell splits into a daughter nucleus and migrates into the daughter cell. The bud continues to grow until it separates from the parent cell, forming a new cell. Some yeasts reproduce by binary fission instead of budding.

Under high stress conditions haploid cells will generally die, however under the same conditions diploid cells can undergo sporulation, entering sexual reproduction (meiosis) and producing a variety of haploid spores, which can go on to mate (conjugate), reforming the diploid.

 

Procedure to observe Amoeba movement and structure

  • Use a dissecting microscope to examine a culture of living Amoeba. Locate individuals on the bottom.
  • Prepare a wet mound to living Amoebas by using an eyedropper to remove a few drops from the bottom of the culture of organisms.
  • Put the drops in a depression slide if one is available or use a standard slide.
  • Cover the preparation with a coverslip and examine under low power (10x). Soon the Amoeba should move by extending their pseudopods.
  • If nutrient broth is available, add a drop to the preparation and observe the Amoeba’s response.


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Answered by  | 12th Oct, 2010, 07:58: PM

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