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ICSE Class 9 Answered

  How to create the  medium for tissue  culture   at  home and how to prepare the medium  
Asked by vsrushil | 24 Jul, 2018, 07:11: PM
answered-by-expert Expert Answer

The ingredients indicated below can be prepared in home/taken easily

  1. Two cups of rain water
  2. A quarter cup of sugar
  3. Fertiliser stock: 1/2 tablespoon all purpose 10:10:10 (N.P.K.) water soluble fertiliser in 1L of water: use one cup of stock for this recipe.
  4. Inositol tablets (500 mg): 1/2 tablet
  5. Vitamin tablet with thiamine: 1/2 tablet- Any multivitamin tablet may be used.
  6. Agar flakes: 4 tablespoons

This is the basic media.

You can also use half a cup of coconut milk and a half teaspoon of malt. Replacing the coconut milk with a half cup of green tomato puree or a half cup of freshly squeezed orange juice may produce different responses. 

Mix the ingredients in a saucepan and gently boil until the agar has dissolved, stirring continuously to stop the agar sticking and subsequently burning at the bottom of the pan. Dispense into empty glass jars so that the medium is about 2 cm deep. Cover and process in a pressure cooker/microwave. Cook for 15 minutes after the pressure is reached.

Answered by Sivanand Patnaik | 24 Jul, 2018, 07:27: PM
ICSE 9 - Biology
Asked by anumoludeepak9923.9sdatl | 27 Apr, 2020, 05:13: PM
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ICSE 9 - Biology
Asked by vsrushil | 24 Jul, 2018, 07:48: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
ICSE 9 - Biology
Asked by vsrushil | 24 Jul, 2018, 07:11: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
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