Archive
7th of September 2015
Biology
Q:

can you explain me the phenomenon of sticky ends how it actually happens Im little confused pls can you help me out.

Jaskamal Kaur - CBSE - Class XII Science

Monday, September 07, 2015 at 19:00:PM

A:

Hi Jaskamal,

  • Special sequences in DNA recognised by restriction endonucleases are called palindromic nucleotide sequences.
  • The palindromes in DNA are base pair sequences which are the same when read forward or backward from a central axis of symmetry.
  • Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases of the opposite strands. This leaves single-stranded unpaired bases at the cut ends.
  • These ends with unpaired bases are called sticky ends or cohesive ends. They form hydrogen bonds with their complementary cut counter parts.
  • The sticky ends facilitate the action of the enzyme DNA ligase.

Monday, September 07, 2015 at 19:03:PM