CBSE Class 12-science Answered
Which property of a colloid is used in electron microscope?
Asked by Kalindi Varma | 14 Feb, 2014, 04:37: AM
Expert Answer
Dear Kalindi,
- Electron dense markers of a size suitable for transmission electron microscopy and scanning electron microscopy have been prepared with gold granules labeled with a monolayer of specific macromolecules.
- Colloidal Gold particles are most often attached to secondary antibodies which are in turn attached to primary antibodies designed to bind a specific protein or other cell component.
- Gold is used for its high electron density which increases electron scatter to give high contrast 'dark spots'.
- Immunogold labelling or Immunogold staining (IGS) is a staining technique used in electron microscopy.
Answered by | 17 Feb, 2014, 03:14: PM
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