CBSE Class 12-science Answered
what is insertional inactivation?
Asked by prativapradhan63 | 20 Jan, 2015, 08:46: PM
Expert Answer
It is technique used in recombinant DNA technology in which a fragment of foreign DNA is inserted into a restriction site within a gene for antibiotic resistance, thus causing the genbe to be inactivated. The best example is pUC19 plasmid vector where the lacZ gene that encodes for beta-galactosidase cannot be longer produced when the foreign gene is inserted to it.
Answered by Sivanand Patnaik | 21 Jan, 2015, 08:09: AM
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