CBSE Class 12-science Answered
Study the diagram given below and
answer the questions that follow:
(a) Why have DNA fragments in band
'D' moved farther away in comparison to those in band 'C'?
(b) Identify the anode end in the diagram.
(c) How are these DNA fragments visualised?
(a) Why have DNA fragments in band
'D' moved farther away in comparison to those in band 'C'?
(b) Identify the anode end in the diagram.
(c) How are these DNA fragments visualised?
Asked by Topperlearning User | 12 Jun, 2014, 09:24: AM
Expert Answer
(a) In electrophoresis, fragments move towards the anode according to their molecular size. The smaller fragments move farther away as compared to larger fragments. DNA fragments in band 'D' are smaller in size than fragments in band 'C'. Therefore, they moved faster and farther away.
(b) The anode end is 'B' as DNA fragments in band 'D' moved farther away towards the ‘B’ end than fragments in band ‘C’.
(c) The separated DNA fragments can be visualised by staining the DNA with ethidium bromide followed by exposure to UV radiation.
Answered by | 12 Jun, 2014, 11:24: AM
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