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PLEASE EXPLAIN CHROMATOGRAPHY IN SIMPPLS WORDS.(WITH EXAMPLE)

Asked by binodbharali 15th June 2016, 7:32 PM
Answered by Expert
Answer:

PRINCIPLE: This method is based on the solubility of different components in solvent. The ink which we use has water as the solvent with the dye dissolved in it. As water rises on the filter paper it carries along with it the dye particles.

The colour component which is more soluble in water rises faster and in this way the colours get separated.

TECHNIQUE:

  • Place a spot of ink with the help of a capillary tube in the centre of a base line, about 2-3 cm away from the lower edge of a paper.
  • Allow the spot to dry and hang it in a glass jar with its lower end immersed in the solvent.

Separation of Compounds

  • The solvent runs over the spot and carries the components to a distance along the paper, indicated by the colored spots.

DIAGRAM:

 

EXAMPLE: Different component dyes in ink.

Pigments from natural colours.

Drugs from blood.

Answered by Expert 16th June 2016, 10:40 AM
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