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how did elililly company extract humulin ? 

Asked by lathapurushotham.91 27th January 2016, 6:22 PM
Answered by Expert

Hi Venkatesh, 

Humulin is synthesized in E.coli using recombinant DNA technology. 

The genes for the production of A and B chain of insulin are incoorperated into the bacterial plasmid and introduced in the bacteria. The transformed bacteria are selected by growing them on selective media and the A and B chain protein for insulin is extracted. The two chains are mixed to obtain a functional insulin protein. 

Answered by Expert 27th January 2016, 6:30 PM
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