CBSE Class 12-science Answered
Explain the importance of (a) ori, (b) ampR and (c) rop in the E. coli vector shown below:
Asked by Topperlearning User | 12 Jun, 2014, 09:24: AM
Expert Answer
(a) ori: Ori or origin of replication is a specific portion of plasmid genome that serves as a start signal for self-replication. Any piece of DNA when linked to this sequence can be made to replicate within the host cells. It is also responsible for controlling the copy number of the linked DNA.
(b) ampR: The ligation of foreign DNA is carried out at a restriction site present in any antibiotic resistance gene. It acts as a selectable marker during transformation.
(c) rop: It codes for the proteins involved in the replication of the plasmid.
Answered by | 12 Jun, 2014, 11:24: AM
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