CBSE - XII Science - Biology
can you explain me the phenomenon of sticky ends how it actually happens Im little confused pls can you help me out.
- Special sequences in DNA recognised by restriction endonucleases are called palindromic nucleotide sequences.
- The palindromes in DNA are base pair sequences which are the same when read forward or backward from a central axis of symmetry.
- Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases of the opposite strands. This leaves single-stranded unpaired bases at the cut ends.
- These ends with unpaired bases are called sticky ends or cohesive ends. They form hydrogen bonds with their complementary cut counter parts.
- The sticky ends facilitate the action of the enzyme DNA ligase.
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