Request a call back

Join NOW to get access to exclusive study material for best results

CBSE Class 12-science Answered

can you explain me pBR322 cycle
Asked by JASKAMAL KAUR | 07 Sep, 2015, 06:52: PM
answered-by-expert Expert Answer

Hi Jaskamal,

  • pBR322 was the first artificial cloning vector constructed by Bolivar and Rodriguez in 1977.
  • The nomenclature of the pBR322 plasmid is as stated below: P – Plasmid, BR - Stands for Bolivar and Rodriguez who constructed this plasmid, 322 - Number given to distinguish this plasmid from the other plasmids developed in the same laboratory.

  • Plasmid pBR322 has two resistance genes—ampicillin resistance (ampR) and tetracycline resistance (tetR)—which are considered useful for selectable markers. Selectable markers help in selecting those host cells which contain the transformed vectors and eliminate the non-transformants.
  • Plasmid pBR322 has a variety of unique recognition sites for restriction endonucleases.
  • Two unique sites—PstI and PvuI—are located within the ampR gene and BamHI and SalI are within the tetR gene.
  • Insertion of the DNA fragment into the plasmid using enzyme PstI or PvuI places the DNA insert within the gene ampR, which makes ampR non-functional.
  • Bacterial cells containing such a recombinant pBR322 will be unable to grow in the presence of ampicillin but will grow on tetracycline. 
Answered by | 07 Sep, 2015, 07:05: PM
CBSE 12-science - Biology
Asked by rounakguita | 19 Mar, 2024, 12:37: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by daminicherom08 | 16 Mar, 2024, 07:44: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by nashifaafroj | 18 Jan, 2024, 11:38: AM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by armanchahar266 | 09 Jan, 2024, 10:52: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by neet14077 | 09 Jan, 2024, 10:43: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by suhailshowkat935 | 31 Dec, 2023, 07:06: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by hm6929460 | 27 Dec, 2023, 08:10: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
CBSE 12-science - Biology
Asked by qureshigaffer | 26 Dec, 2023, 12:06: PM
ANSWERED BY EXPERT ANSWERED BY EXPERT
Get Latest Study Material for Academic year 24-25 Click here
×