Why is it essential to have a selectable marker in a cloning vector?
Asked by pksunilnair | 12th Dec, 2017, 08:50: PM
Selectable markers are essential to identify and eliminate non-transformants(no recombinant DNA), and selectively permitting the growth of the transformants (host cells bearing recombinant DNA).
Answered by Sivanand Patnaik | 13th Dec, 2017, 08:38: AM
- recombinant DNA tecnology
- please answer
- What is ori? state its importance during cloning of a vector?
- What is the host that produces a foreign gene product called? What is this product called?
- State the uses of cloning vector in biotechnology.
- Who developed the technique of electrophoresis? State its principle.
- Explain any two methods of vectorless gene transfer.
- What is genetic engineering? List the steps involved in rDNA technology.
- How can DNA segments be separated by gel electrophoresis, visualised and isolated?
- Study the linking of DNA fragments shown below: (i) Name 'a' DNA and 'b' DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments.
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