What is RFLP and what is its work?

Asked by Areeba Ahmad | 15th Mar, 2015, 11:57: PM

Expert Answer:

Restriction Fragment Length Polymorphism (RFLP) is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples with specific restriction endonucleases. RFLP, as a molecular marker, is specific to a single clone/restriction enzyme combination.

The RFLP probes are frequently used in genome mapping and also finds use in genotyping, forensics, paternity tests, hereditary disease diagnostics, etc.

Answered by Sivanand Patnaik | 16th Mar, 2015, 09:48: AM