What is a black reaction?

How was Camilio Golgi able to identify it

Asked by mukunda | 2nd Jan, 2020, 09:40: PM

Expert Answer:

  • Black reaction is a silver staining technique which was first done by Camillo Golgi. It helped to study about the nerve cells. In this technique, cells are stained using potassium dichromate and silver nitrate.
  • In 1873, Camillo Golgi used the chemical potassium dichromate to harden the tissue.
  • However, after hardening the tissue, Golgi used a different chemical to dye the neurons. Instead of hematoxylin or carmine, Golgi submerged the tissue in a silver nitrate solution, soaking it for one to two days.
  • The silver nitrate solution reacted with the potassium dichromate and formed fragments of silver chromate on the cell membrane. Those fragments were black, hence the name black reaction.

Answered by Sheetal Kolte | 3rd Jan, 2020, 06:15: PM