What are recombinant DNA and transformable &non transformant DNA?
Asked by bijuaylara111 | 21st Oct, 2017, 09:20: AM
Recombinant DNA, or rDNA, is the term used to describe the combination of two DNA strands that are constructed artificially. Transformable DNA receives either a recombinant or non-recombinant plasmid whereas non-transformanrt DNA do not receive any plasmid.
Answered by Sivanand Patnaik | 21st Oct, 2017, 07:06: PM
- What is ori? state its importance during cloning of a vector?
- What is the host that produces a foreign gene product called? What is this product called?
- State the uses of cloning vector in biotechnology.
- Who developed the technique of electrophoresis? State its principle.
- Explain any two methods of vectorless gene transfer.
- What is genetic engineering? List the steps involved in rDNA technology.
- How can DNA segments be separated by gel electrophoresis, visualised and isolated?
- Study the linking of DNA fragments shown below: (i) Name 'a' DNA and 'b' DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments.
- EcoRI is used to cut a segment of foreign DNA and that of a vector DNA to form recombinant DNA. Show with the help of schematic diagrams. (i) The set of palindromic nucleotide sequence of base pairs EcoRI will recognise in both the DNA segments. Mark the site at which EcoRI will act and cut both the segments. (ii) Sticky ends formed on both the segments where the two DNA segments will join later to form a recombinant DNA.
- (a) What are molecular scissors? Give one example. (b) Explain their role in recombinant DNA technology.
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