CBSE Class 12-science Answered
Differences between DNA polymerase I, II and III in prokaryotes:
Point of Difference |
DNA Polymerase I |
DNA Polymerase II |
DNA Polymerase III |
Structural gene |
pol A |
pol B |
pol C |
Number of subunits in the structure |
1 |
Greater than or equal to 4 |
Greater than or equal to 10 |
Rate of Polymerisation |
Low (16-20 nucleotides/sec) |
Low (40 nucleotides/sec) |
High (250-1000 nucleotides/sec) |
Processivity (nucleotides added before polymerase dissociates) |
Low (3-200) |
Low (1500) |
High Greater than or equal to 5,00,000 |
Proof-reading activity |
3ˈ-5ˈ and 5ˈ-3ˈ exonuclease activities |
3ˈ-5ˈ exonuclease activity |
3ˈ-5ˈ exonuclease activity |
Efficiency of primer removal |
Best |
Nil |
Nil |
Type of strand synthesised |
Lagging strand |
No role in strand synthesis |
Leading and lagging strands |
Role in DNA repair |
Active |
Active |
No role |
Biological functions in the cell |
DNA replication, Processing of Okazaki fragments, maturation Excision repair |
DNA replication, DNA repair, Translesion DNA synthesis
|
DNA replication, DNA repair
|