How can I perform the onion peel experiment best?

Asked by happy1996 | 18th Jul, 2010, 12:00: AM

Expert Answer:

 

Take a small piece from an onion bulb and peel off the thin transparent epidermis, called onion peel, from the concave side of the onion. Take some water in a watch glass and put the peel immediately in it. Make certain that it is not folded or rolled.

Add 1-2 drops of Iodine solution or Safranine solution.

After about 2-3 minutes take the peel and wash in acid water if iodine solution is used or in plain water if safranine is used.

Take a clean glass slide and put a drop of glycerine in the middle of the slide.

Place the stained piece of the peel in the glycerine. A thin camel hair paintbrush might be necessary to help transfer the peel. Then put a cover slip on it with the help of a mounting needle. Take care to avoid air bubbles while putting the cover slip.

Soak the overflowing fluid from the corners of the coverslip with the help of the blotting paper and clean the side.

Observe the wet mount under the low power of the microscope and then under high power.

Answered by  | 19th Jul, 2010, 03:55: PM

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