How can DNA segments be separated by gel electrophoresis, visualised and isolated?

Asked by Topperlearning User | 12th Jun, 2014, 09:24: AM

Expert Answer:

As pure DNA fragments cannot be seen under visible light, they can be visualised only after staining them with a solution of ethidium bromide followed by exposure to UV radiation. They appear as bright orange coloured bands. The separated bands of DNA are then cut from the agarose gel and extracted by using a convenient technique. This process is called elution. The eluted DNA fragments are then purified and used in constructing recombinant DNA by joining them with cloning vectors.

Answered by  | 12th Jun, 2014, 11:24: AM