How can DNA segments be separated by gel electrophoresis, visualised and isolated?
Asked by Topperlearning User | 12th Jun, 2014, 09:24: AM
As pure DNA fragments cannot be seen under visible light, they can be visualised only after staining them with a solution of ethidium bromide followed by exposure to UV radiation. They appear as bright orange coloured bands. The separated bands of DNA are then cut from the agarose gel and extracted by using a convenient technique. This process is called elution. The eluted DNA fragments are then purified and used in constructing recombinant DNA by joining them with cloning vectors.
Answered by | 12th Jun, 2014, 11:24: AM
- recombinant DNA tecnology
- please answer
- What is ori? state its importance during cloning of a vector?
- What is the host that produces a foreign gene product called? What is this product called?
- State the uses of cloning vector in biotechnology.
- Who developed the technique of electrophoresis? State its principle.
- Explain any two methods of vectorless gene transfer.
- What is genetic engineering? List the steps involved in rDNA technology.
- Study the linking of DNA fragments shown below: (i) Name 'a' DNA and 'b' DNA. (ii) Name the restriction enzyme that recognises this palindrome. (iii) Name the enzyme that can link these two DNA fragments.
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