CBSE Class 12-science Answered
How and why is bacterium Thermus aquaticus employed in
recombinant DNA technology? Explain.
Asked by Topperlearning User | 10 Jun, 2014, 05:28: PM
Expert Answer
DNA polymerase is obtained from the bacterium, Thermus aquaticus.
(i) DNA polymerase obtained from this organism (Taq DNA polymerase) is thermostable and remains active during high temperature applied during the denaturation of double-stranded DNA.
(ii) This enzyme extends the primers using nucleotides provided in the reaction and the genomic DNA as template.
(iii) The repeated amplification is achieved by this enzyme and the amplified fragment, if desired can be used to ligate with a vector for further cloning.
Answered by | 10 Jun, 2014, 07:28: PM
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